Nutritional Genomics and Genetics

MSc Course Nutritional Genomics and Genetics (HNE-34804)

March - April 2009

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition"
Expected knowledge on "Cell biology 2", "Genomics"
Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"

The lectures are in room C8 (Tuesday and Wednesday) or C 221 (Thursday)

A syllabus (with handouts and papers) will be sold in the course.

			Zaal	Gebouw	Activiteit	Lecturer
Wk 26	Ma 2-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs	Intro Michael Muller Case I/1	Müller Busstra
	Di 3-3	13.30-15.15	C8	Scheikundegebouw, coll zaal 70 pers	Concept & Objectives of the Course What is Nutrigenomics and what is molecular nutrition research?	Müller
	Woe 4-3	15.30-18.00	C8	Scheikundegebouw, coll zaal 70 pers	Genomics tools overview Assignment research proposal Assignment background knowledge (self study)	Lichtenauer
	Do 5-3	13.30-17.15	PC55, PC411,PC610	Technotron 21 PCs 40 pers/ Forum 11 PCs 22 pers/ Forum 11 PCs 22 pers	eLearning Proteomics	Lichtenauer+ aio’s
	Vrij 6-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Continue Proteomics Questions background knowledge	Lichtenauer

Wk27	Ma 9-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Case I/2	Busstra
	Di 10-3	13.30-15.15	C8	Scheikundegebouw, coll zaal 70 pers	Genomics Tools 1: Transcriptomics	Boekschoten
	Woe 11-3	15.30-18.00	C8	Scheikundegebouw, coll zaal 70 pers	Nutrient sensors: Transcription factors and Dietary Signaling Routes	Müller
	Do 12-3	13.30-15.15	C221	Forum, coll zaal 72 pers	Use of animal models for nutritional studies	Hooiveld
	Vrij 13-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Group work proposal Paper assignments	Lichtenauer +aio’s

Wk28	Ma 16-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Case II/1	Busstra
	Di 17-3	13.30-15.15	C8	Scheikundegebouw, coll zaal 70 pers	Nutrigenetics and Sensitizing Genotypes	Müller
	Woe 18-3	15.30-18.00	C8	Scheikundegebouw, coll zaal 70 pers	Human nutrigenomics studies	Afman
	Do 19-3	13.30-15.15	C221	Forum, coll zaal 72 pers	Complex genetics, complex phenotypes and diseases	Müller
	Vrij 20-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Group work proposal Paper assignments	Lichtenauer +aio’s

Wk29	Ma 23-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Case II/2	Busstra
	Di 24-3	13.30-15.15	C8	Scheikundegebouw, coll zaal 70 pers	Complex diseases and Nutrition: Genetic Epidemiology	Feskens
	Woe 25-3	15.30-18.00	C8	Scheikundegebouw, coll zaal 70 pers	The Role of Inflammation in the pathophysiology of complex diseases	Müller
	Do 26-3	13.30-15.15	C221	Forum, coll zaal 72 pers	Epigenetics and nutritional epigenomics	Müller
	Vrij 27-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Group work proposal Paper assignments	Lichtenauer +aio’s

Wk30	Ma 30-3	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC
	Di 31-3	13.30-17.15	PC22	Computechnion, 30 PCs 60 pers	The Nutrigenomics Data Set	Afman+aio’s
	Woe 1-4	13.30-17.15	PC22	Computechnion, 30 PCs 60 pers	The Analysis	Afman+aio’s
	Do 2-4	13.30-17.15	PC21, PC411,PC610	Computechnion 13 PCs 26 pers/ Forum 11 PCs 22 pers/ Forum 11 PCs 22 pers	Data Mining 1	Afman+aio’s
	Vrij 3-4	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Data Mining 2 / Pathway Reconstruction	Afman+aio’s

Wk30	Ma 30-3	13.30-17.15	P75	Biotechnion, prakticumzaal 30 pers	Introduction practical course Tissue culture (in groups)	Laetitia, Maryam, Mechteld, aio’s
	Di 31-3	13.30-17.15	P75	Biotechnion, prakticumzaal 30 pers	RNA isolation and EtOH purification	Laetitia, Maryam, Mechteld, aio’s
	Woe 1-4	13.30-17.15	P75	Biotechnion, prakticumzaal 30 pers	cDNA synthesis	Laetitia, Maryam, Mechteld, aio’s
	Do 2-4	13.30-17.15	P75	Biotechnion, prakticumzaal 30 pers	Real time PCR	Laetitia, Maryam, Mechteld, aio’s
	Vrij 3-4	13.30-17.15	P75	Biotechnion, prakticumzaal 30 pers	Real time PCR	Laetitia, Maryam, Mechteld, aio’s

Wk 31	Ma 6-4	13.30-17.15	PC713, PC717	Forum 23+28 PCs, beamer+PC	Case III	Busstra
	Di 7-4	13.30-15.15	C8	Scheikundegebouw, coll zaal 70 pers	Nutrigenomics and Systems Biology	Müller
	Woe 8-4	15.30-18.00	C8	Scheikundegebouw, coll zaal 70 pers	Nutrigenomics Projects: Presentations Students	Müller
	Do 9-4	13.30-15.15	C221	Forum, coll zaal 72 pers	Nutrigenomics & the food industry - Future Perspectives and Conclusions	Witkamp/ Müller
	Vrij 10-4				Goede Vrijdag

Wk 31	Ma 6-4
	Di 7-4	13.30-17.15	PC22	Computechnion, 30 PCs 60 pers	15.30-17.15 na college
	Woe 8-4	13.30-17.15	PC22	Computechnion, 30 PCs 60 pers	13.30-15.00 voor presentaties
	Do 9-4
	Vrij 10-4				Goede Vrijdag

Wk 32	Studieweek
Wk 33	Vrij 24-4 Tentamen

Aims: After the course, the student will
- understand the basics of genetics, genomics and gene regulation with relation to diet
- be able to read and understand literature of the field (molecular nutrition and Nutrigenomics)
- have good understanding of the concepts of molecular nutrition research (signals and signaling pathways, dietary sensors (nuclear receptors), organ sensing, use of animal models)
- have some understanding of the concepts of Nutrigenetics (genetic susceptibility, SNPs, polygenic (complex) diseases, "personalized" diet);
- have good understanding of the concepts of Nutrigenomics (dietary signatures, identification of very early biomarkers, "Two Hits");
- have some (practical) knowledge to apply molecular nutrition and nutrigenomics in the lab (Use of transgenic animals and cells, RNA interference, quantitative PCR, promoter assays, chromatin immunoprecipitation, oligonucleotide and protein microarrays, bioinformatics, biostatistics, pathway mining programs);
- be able to extract relevant data/information from internet for molecular nutrition research;
- be able to plan a "Nutrigenomics" experiment.

Activities: To reach these objectives, the student will
- prepare and follow lectures;
- follow the e-learning modules and solve the cases presented there;
- search the literature (bib) and the www (internet) and read and discuss relevant original research papers (group work), and prepare a presentation;
- discuss ideas and concepts with other students and coaches/teachers;
- use the Blackboard site

Examination: written examination (individual) + performance during presentation (individual);

Literature: Syllabus (you have to buy; includes the handouts + additional resources (reviews, papers etc))

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition" Expected knowledge on "Cell biology 2", "Genomics" Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition"
Expected knowledge on "Cell biology 2", "Genomics"
Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"