MSc Course Nutritional Genomics and Genetics (HNE-34804)

March - April 2008

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition"
Expected knowledge on "Cell biology 2", "Genomics"
Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"

The lectures are in room C8 (Tuesday and Wednesday) or C 221 (Thursday)

A syllabus (with handouts and papers) will be sold in the course.

There will be also an e-learning and a practical part.

 

Date

Time

Topic

Teacher

Week 1

e-learning 1

3-3

1:30-5:00

Case I/1(bring your headphones with you)

Busstra

lecture 1

4-3

1:30-3:00

Concept & Objectives of the Course
Diet, Nutrition and Nutrients: The signals
What is Nutrigenomics ?

Müller

lecture 2

5-3

3:30-6:00

Nutrigenomics: Molecular Nutrition Research

Müller

lecture 3

6-3

1:30-3:00

Use of Models in Animal and Human Studies

Hooiveld

Week 2

e-learning 2

10-3

1:30-5:00

Case I/2 (headphones!!)

Busstra

lecture 4

11-3

1:30-3:00

Nutrient sensors: Transcription factors and Dietary Signaling Routes

Müller

lecture 5

12-3

3:30-6:00

Genomics Tools – How to use them in Nutrigenomics Research?
Transcriptomics / Proteomics

Boekschoten
Lichtenauer

lecture 6

13-3

1:30-3:00

Nutrigenetics and Sensitizing Genotypes

Müller

Week 3

e-learning 3

17-3

1:30-5:00

Case II/1(headphones !!)

Busstra

lecture 7

18-3

1:30-3:00

Complex diseases: Obesity

Müller

lecture 8

19-3

3:30-6:00

Complex diseases: Metabolic Syndrome

Müller

lecture 9

20-3

1:30-3:00

Complex diseases: Diabetes Type 2

Müller

Week 4

lecture 10

25-3

1:30-3:00

Complex diseases and Nutrition: Genetic Epidemiology

Feskens

lecture 11

26-3

3:30-6:00

Complex diseases: The Role of Inflammation and the Two Hits

Müller

lecture 12

27-3

1:30-3:00

Nutrigenomics and Systems Biology

Müller

Week 5

e-learning 4

31-3

1:30-5:00

Case II/2 (headphones !!)

Busstra

lab
practice

 

 

 

Introduction practical course

 

 

 

Tissue culture (in groups)

L. Lichtenstein

 

1-4

1.30-5.00

RNA isolation and EtOH purification

2-4

1.30-5.00

cDNA synthesis

3-4

1.30-5.00

Real time PCR

4-4

1.30-5.00

Real time PCR

bioinformatics
practice

1-4

1.30-5.00

The Nutrigenomics Data

L. Afman

2-4

1.30-5.00

The Analysis

3-4

1.30-5.00

Data Mining 1

4-4

1.30-5.00

Data Mining 2 / Pathway Reconstruction

Week 6

e-learning 5

7-4

1:30-5:00

Case III (bring your headphones with you)

Busstra

lecture 13

8-4

1:30-3:00

Presentations Students

Müller

lecture 14

9-4

3:30-6:00

Nutrigenomics: Functional foods and Personalized Diets
Presentations Students

Müller

lecture 15

10-4

1:30-3:00

Nutrigenomics & the Industry - Future Perspectives and Conclusions

Müller

Aims: After the course, the student will
- understand the basics of genetics, genomics and gene regulation with relation to diet;

- be able to read and understand literature of the field (molecular nutrition and nutrigenomics);
- have good understanding of the concepts of molecular nutrition research (signals and signaling pathways, dietary sensors (nuclear receptors), organ sensing, use of animal models);
- have some understanding of the concepts of nutrigenetics (genetic susceptibility, SNPs, polygenic (complex) diseases, "personalized" diet);
- have good understanding of the concepts of nutrigenomics (dietary signatures, identification of very early biomarkers, "Two Hits");
- have some (practical) knowledge to apply molecular nutrition and nutrigenomics in the lab (Use of transgenic animals and cells, RNA interference, quantitative PCR, promoter assays, chromatin immunoprecipitation, oligonucleotide and protein microarrays, bioinformatics, biostatistics, pathway mining programs);
- be able to extract relevant data/information from internet for molecular nutrition research;
- be able to plan a "Nutrigenomics" experiment
.

Activities: To reach these objectives, the student will
- prepare and follow lectures;

- follow the e-learning modules and solve the cases presented there;
- search the literature (bib) and the www (internet) and read and discuss relevant original research papers (group work), and prepare a presentation;
- discuss ideas and concepts with other students and coaches/teachers;

- use the Blackboard site

Examination: written examination (individual) + performance during presentation (individual);

Literature: Syllabus (you have to buy; includes the handouts + additional resources (reviews, papers etc))

More on Blackboard