MSc Course Nutritional Genomics and Genetics (HNE-34804) March - April 2010

The lectures are in room C8 (Tuesday and Wednesday) or C 221 (Thursday)

Rooms are: PC713, PC717 (Forum), C8 (Chemistry building), C221 (Forum), PC21, PC22 (Computechnion), P57 (Erfelijkheidsleer)

A syllabus (with handouts and papers) will be sold in the course.

Date

Time

Location

Lecture

Activity

Lecturer

 

 

 

 

Week 1 (What is the problem?)

 

Mon 1-3

13.30-17.15

PC713, PC717

 

eLearning Case I/1 (Intro M. Müller)

Rakhshandehroo

Tue 2-3

13.30-15.15

C8

NGG1

Concept & Objectives of the Course
What is Nutrigenomics & molecular nutrition research?

Müller

Wed 3-3

15.30-18.00

C8

NGG2

Genomics 1: Overview / From Genotype to Phenotype
How to measure the nutritional phenotype?

Müller

Thu 4-3

13.30-17.15

C221

NGG3

Genomics 2: Genomics & Transcriptomics

Müller

Fri 5-3

13.30-17.15

 

 

Group work, Paper assignments

 

 

 

 

 

Week 2 (What are the tools?)

 

Mon 8-3

13.30-17.15

PC713, PC717

 

eLearning Case I/2

Rakhshandehroo

Tue 9-3

13.30-15.15

C8

NGG4

Genomics 3: Proteomics & Metabolomics / Applications of model systems

Müller

Wed 10-3

15.30-18.00

C8

NGG5

Nutrient Sensing: The role of Sensing Transcription factors and Dietary Signaling Routes

Müller

Thu11-3

13.30-15.15

C221

NGG6

Nutrient Sensing in organs / Role of intestine, liver, adipose tissue and PBMCs

Müller

Fri12-3

13.30-17.15

 

 

Group work, Paper assignments

 

 

 

 

 

Week 3 (From Genotype to Phenotype)

 

Mon 15-3

13.30-17.15

PC713, PC717

 

eLearning Case II/1

Rakhshandehroo

Tue 16-3

13.30-15.15

C8

NGG7

Nutrition, Genetics and Sensitizing Genotypes

Müller

Wed 17-3

15.30-18.00

C8

NGG8

Complex diseases: GWAS

Müller

Thu 18-3

13.30-15.15

C221

NGG9

Complex diseases: From Genotype to Phenotype

Müller

Fri 19-3

13.30-17.15

 

 

Group work, Paper assignments

 

 

 

 

 

Week 4 (From Health to Diseases)

 

Mon 22-3

13.30-17.15

PC713, PC717

 

eLearning Case II/2

Rakhshandehroo

Tue 23-3

13.30-15.15

C8

NGG10

Epigenetics and nutritional epigenomics

Müller

Wed 24-3

15.30-18.00

C8

NGG11

The role of the intestine as gatekeeper (Host, microbes, foods)

Müller

Thu25-3

13.30-15.15

C221

NGG12

The Role of inflammation in the pathophysiology of complex diseases

Müller

Fri 26-3

13.30-17.15

 

 

Group work proposal / Paper assignments

 

 

 

 

 

Week 5 (Practical Part Dry Lab) Group A

 

Mon 29-3

13.30-17.15

PC713, PC717

 

The Nutrigenomics Data Set

Afman

Tue 30-3

13.30-17.15

PC22

 

The Analysis

Afman

Wed 31-3

13.30-17.15

PC22

 

Data Mining 1

Afman

Thu 1-4

13.30-17.15

PC21, PC411, PC610 (Forum)

 

Data Mining 2 / Pathway Reconstruction

Afman

Fri 2-4

 

 

 

Goede Vrijdag (holiday)

 

 

 

 

 

Week 5 (Practical Part Wet Lab) Group B

 

Mon 29-3

13.30-17.15

P57

 

Introduction practical course / Tissue culture (in groups)

 

Tue 30-3

13.30-17.15

P57

 

RNA isolation and EtOH purification

 

Wed 31-3

13.30-17.15

P57

 

cDNA synthesis

 

Thu 1-4

13.30-17.15

P57

 

Real time PCR

 

Fri 2-4

 

 

 

Goede Vrijdag (holiday)

 

 

 

 

 

Week 6 (Applications of Nutrigenomics)

 

Mon 5-4

Free

 

 

 

 

Tue 6-4

13.30-15.15

C8

NGG13

Nutritional Systems Biology & Nutritional Science 2.0

Müller

Wed 7-4

13.30-17.15

C8

NGG14

Examples of actual nutrigenomics projects; PhD students present their projects

PhD students, Hooiveld

Thu 8-4

 

C221

NGG15

The role of the food industry: Functional Foods, EFSA & Health Claims

Witkamp

 

 

 

 

 

 

Tue 14-4 –Fri 17-4

 

 

 

Week 7 Study week

 

Fri 24-4

 

 

 

Week 8 Exam

 

Aims: After the course, the student will
 - understand the basics of genetics, genomics and gene regulation with relation to diet
- be able to read and understand literature of the field (molecular nutrition and Nutrigenomics)
 - have good understanding of the concepts of molecular nutrition research (signals and signaling pathways, dietary sensors (nuclear receptors), organ sensing, use of animal models)
- have some understanding of the concepts of Nutrigenetics (genetic susceptibility, SNPs, polygenic (complex) diseases, "personalized" diet);
- have good understanding of the concepts of Nutrigenomics (dietary signatures, identification of very early biomarkers, "Two Hits");
- have some (practical) knowledge to apply molecular nutrition and nutrigenomics in the lab (Use of transgenic animals and cells, RNA interference, quantitative PCR, promoter assays, chromatin immunoprecipitation, oligonucleotide and protein microarrays, bioinformatics, biostatistics, pathway mining programs);
- be able to extract relevant data/information from internet for molecular nutrition research;
- be able to plan a "Nutrigenomics" experiment.

Activities: To reach these objectives, the student will
- prepare and follow lectures;
- follow the e-learning modules and solve the cases presented there;
- search the literature (bib) and the www (internet) and read and discuss relevant original research papers (group work), and prepare a presentation;
- discuss ideas and concepts with other students and coaches/teachers;
- use the Blackboard site

Examination: written examination (individual) + performance during presentation (individual);

Literature: Syllabus (you have to buy; includes the handouts + additional resources (reviews, papers etc))

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