Nutritional Genomics and Genetics

MSc Course Nutritional Genomics and Genetics (HNE-34804)

March - April 2008

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition"
Expected knowledge on "Cell biology 2", "Genomics"
Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"

The lectures are in room C8 (Tuesday and Wednesday) or C 221 (Thursday)

A syllabus (with handouts and papers) will be sold in the course.

There will be also an e-learning and a practical part.

	Date	Time	Topic	Teacher
Week 1
e-learning 1	3-3	1:30-5:00	Case I/1(bring your headphones with you)	Busstra
lecture 1	4-3	1:30-3:00	Concept & Objectives of the Course Diet, Nutrition and Nutrients: The signals What is Nutrigenomics ?	Müller
lecture 2	5-3	3:30-6:00	Nutrigenomics: Molecular Nutrition Research	Müller
lecture 3	6-3	1:30-3:00	Use of Models in Animal and Human Studies	Hooiveld
Week 2
e-learning 2	10-3	1:30-5:00	Case I/2 (headphones!!)	Busstra
lecture 4	11-3	1:30-3:00	Nutrient sensors: Transcription factors and Dietary Signaling Routes	Müller
lecture 5	12-3	3:30-6:00	Genomics Tools – How to use them in Nutrigenomics Research? Transcriptomics / Proteomics	Boekschoten Lichtenauer
lecture 6	13-3	1:30-3:00	Nutrigenetics and Sensitizing Genotypes	Müller
Week 3
e-learning 3	17-3	1:30-5:00	Case II/1(headphones !!)	Busstra
lecture 7	18-3	1:30-3:00	Complex diseases: Obesity	Müller
lecture 8	19-3	3:30-6:00	Complex diseases: Metabolic Syndrome	Müller
lecture 9	20-3	1:30-3:00	Complex diseases: Diabetes Type 2	Müller
Week 4
lecture 10	25-3	1:30-3:00	Complex diseases and Nutrition: Genetic Epidemiology	Feskens
lecture 11	26-3	3:30-6:00	Complex diseases: The Role of Inflammation and the Two Hits	Müller
lecture 12	27-3	1:30-3:00	Nutrigenomics and Systems Biology	Müller
Week 5
e-learning 4	31-3	1:30-5:00	Case II/2 (headphones !!)	Busstra
lab practice			Introduction practical course
			Tissue culture (in groups)	L. Lichtenstein
	1-4	1.30-5.00	RNA isolation and EtOH purification
	2-4	1.30-5.00	cDNA synthesis
	3-4	1.30-5.00	Real time PCR
	4-4	1.30-5.00	Real time PCR
bioinformatics practice	1-4	1.30-5.00	The Nutrigenomics Data	L. Afman
	2-4	1.30-5.00	The Analysis
	3-4	1.30-5.00	Data Mining 1
	4-4	1.30-5.00	Data Mining 2 / Pathway Reconstruction
Week 6
e-learning 5	7-4	1:30-5:00	Case III (bring your headphones with you)	Busstra
lecture 13	8-4	1:30-3:00	Presentations Students	Müller
lecture 14	9-4	3:30-6:00	Nutrigenomics: Functional foods and Personalized Diets Presentations Students	Müller
lecture 15	10-4	1:30-3:00	Nutrigenomics & the Industry - Future Perspectives and Conclusions	Müller

Aims: After the course, the student will
- understand the basics of genetics, genomics and gene regulation with relation to diet;
- be able to read and understand literature of the field (molecular nutrition and nutrigenomics);
- have good understanding of the concepts of molecular nutrition research (signals and signaling pathways, dietary sensors (nuclear receptors), organ sensing, use of animal models);
- have some understanding of the concepts of nutrigenetics (genetic susceptibility, SNPs, polygenic (complex) diseases, "personalized" diet);
- have good understanding of the concepts of nutrigenomics (dietary signatures, identification of very early biomarkers, "Two Hits");
- have some (practical) knowledge to apply molecular nutrition and nutrigenomics in the lab (Use of transgenic animals and cells, RNA interference, quantitative PCR, promoter assays, chromatin immunoprecipitation, oligonucleotide and protein microarrays, bioinformatics, biostatistics, pathway mining programs);
- be able to extract relevant data/information from internet for molecular nutrition research;
- be able to plan a "Nutrigenomics" experiment.

Activities: To reach these objectives, the student will
- prepare and follow lectures;
- follow the e-learning modules and solve the cases presented there;
- search the literature (bib) and the www (internet) and read and discuss relevant original research papers (group work), and prepare a presentation;
- discuss ideas and concepts with other students and coaches/teachers;
- use the Blackboard site

Examination: written examination (individual) + performance during presentation (individual);

Literature: Syllabus (you have to buy; includes the handouts + additional resources (reviews, papers etc))

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition" Expected knowledge on "Cell biology 2", "Genomics" Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"

Assumed knowledge on "Advanced Metabolic Aspects of Nutrition"
Expected knowledge on "Cell biology 2", "Genomics"
Continuation courses Major/minor "Nutrigenomics" and "Molecular Epidemiology"